LOCUS       sequence-148799-       10254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing PrM from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10254)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_PrM_Flag
FEATURES             Location/Qualifiers
     source          1..10254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3192..3215
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3264..3836
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3431..3448)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3658..3677
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3839..4441
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4338..4358
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4457..5045
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4510..4530)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4928..4939)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5572..5752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5778..5795)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5784..6008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6054..6482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6141..6160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6351..6372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6491..6511)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6496..6825
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6676..6811
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6738..6757
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6873..6920
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6939..7313
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7443..7564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7480..7499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7534..7553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7613..7629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7613..7629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7626..7648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7637..7653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7661..7667,7668..7685,7686..7691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7706..7727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7844..7861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8015..8603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8095..8114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8774..9565,9566..9634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9397..9416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9635..9739)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9814..9833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10005..10254,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtca ccatggcgga ggtcactaga cgtgggagtg
     2701 catactatat gtacttggac agaaacgatg ctggggaggc catatctttt ccaaccacat
     2761 tggggatgaa taagtgttat atacagatca tggatcttgg acacatgtgt gatgccacca
     2821 tgagctatga atgccctatg ctggatgagg gggtggaacc agatgacgtc gattgttggt
     2881 gcaacacgac gtcaacttgg gttgtgtacg gaacctgcca tcacaaaaaa ggtgaagcac
     2941 ggagatctag aagagctgtg acgctcccct cccattccac caggaagctg caaacgcggt
     3001 cgcaaacctg gttggaatca agagaataca caaagcactt gattagagtc gaaaattgga
     3061 tattcaggaa ccctggcttc gcgttagcag cagctgccat cgcttggctt ttgggaagct
     3121 caacgagcca aaaagtcata tacttggtca tgatactgct gattgccccg gcatacagct
     3181 ccgggatggc cgactacaaa gacgacgatg acaagtagca ctcgaggata tcgacgcgtg
     3241 aagcttagcg gccgcaaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     3301 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3361 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3421 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3481 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3541 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     3601 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     3661 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     3721 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     3781 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     3841 ggccaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     3901 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     3961 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4021 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4081 gagcgtcgaa gcgggggcgg tgttcgccga gatcggctcg cgcatggccg agttgagcgg
     4141 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4201 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4261 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gctggggtgc ccgccttcct
     4321 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4381 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4441 agttcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4501 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4561 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4621 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4681 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4741 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4801 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     4861 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4921 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4981 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5041 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     5101 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5161 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5221 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5281 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     5341 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     5401 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     5461 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     5521 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     5581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     5761 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5821 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5881 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5941 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6001 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6061 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6121 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6181 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6241 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6301 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6361 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6421 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6481 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6541 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6661 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6721 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6781 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6841 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     6901 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     6961 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     7021 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     7081 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     7141 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     7201 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     7261 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     7321 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7381 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7441 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7501 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7561 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7621 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7681 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7741 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7801 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9781 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9841 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9901 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9961 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
    10021 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
    10081 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
    10141 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
    10201 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atca
//
