LOCUS       sequence-148800-       10251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing PrM from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10251)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_Flag_PrM
FEATURES             Location/Qualifiers
     source          1..10251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2679..2702
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3261..3833
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3428..3445)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3655..3674
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3836..4438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4335..4355
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4454..5042
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4507..4527)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4925..4936)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5569..5749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5775..5792)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5781..6005
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6051..6479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6138..6157)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6348..6369
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6488..6508)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6493..6822
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6673..6808
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6735..6754
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6870..6917
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6936..7310
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7440..7561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7477..7496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7531..7550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7610..7626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7610..7626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7623..7645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7634..7650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7658..7664,7665..7682,7683..7688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7703..7724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7841..7858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8012..8600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8092..8111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8771..9562,9563..9631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9394..9413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9632..9736)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9811..9830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10002..10251,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtca ccatggccga ctacaaagac gacgatgaca
     2701 agtccggggc ggaggtcact agacgtggga gtgcatacta tatgtacttg gacagaaacg
     2761 atgctgggga ggccatatct tttccaacca cattggggat gaataagtgt tatatacaga
     2821 tcatggatct tggacacatg tgtgatgcca ccatgagcta tgaatgccct atgctggatg
     2881 agggggtgga accagatgac gtcgattgtt ggtgcaacac gacgtcaact tgggttgtgt
     2941 acggaacctg ccatcacaaa aaaggtgaag cacggagatc tagaagagct gtgacgctcc
     3001 cctcccattc caccaggaag ctgcaaacgc ggtcgcaaac ctggttggaa tcaagagaat
     3061 acacaaagca cttgattaga gtcgaaaatt ggatattcag gaaccctggc ttcgcgttag
     3121 cagcagctgc catcgcttgg cttttgggaa gctcaacgag ccaaaaagtc atatacttgg
     3181 tcatgatact gctgattgcc ccggcataca gctagcactc gaggatatcg acgcgtgaag
     3241 cttagcggcc gcaaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     3301 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     3361 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     3421 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     3481 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     3541 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     3601 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     3661 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     3721 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     3781 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggc
     3841 caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     3901 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     3961 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4021 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4081 cgtcgaagcg ggggcggtgt tcgccgagat cggctcgcgc atggccgagt tgagcggttc
     4141 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4201 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4261 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga
     4321 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4381 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
     4441 tcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4501 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4561 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4621 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4681 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4741 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4801 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     4861 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4921 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4981 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5041 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5101 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5161 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5221 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5281 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     5341 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     5401 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     5461 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     5521 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     5581 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5641 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5701 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     5761 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5821 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5881 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5941 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6001 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6061 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6121 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6181 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6241 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6301 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6361 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6421 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     6481 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6541 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6601 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6661 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6721 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     6781 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     6841 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     6901 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     6961 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7021 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     7081 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     7141 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     7201 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     7261 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     7321 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7381 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7441 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7501 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7561 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7621 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7681 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7741 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7801 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7861 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7921 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7981 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8041 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8101 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8161 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8221 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8281 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8341 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8401 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8461 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8521 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8581 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8641 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8701 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8761 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8821 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8881 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8941 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9001 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9061 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9121 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9181 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9241 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9301 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9361 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9421 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9481 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9541 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9601 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9661 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9721 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     9781 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9841 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     9901 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     9961 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
    10021 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
    10081 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    10141 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    10201 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc a
//
