LOCUS       sequence-148802-       11598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing NS3 from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11598)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 11598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_Flag_NS3
FEATURES             Location/Qualifiers
     source          1..11598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2679..2702
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4608..5180
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4775..4792)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5002..5021
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5183..5785
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5682..5702
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5801..6389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5854..5874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6272..6283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6916..7096
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7122..7139)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7128..7352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7398..7826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7485..7504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7695..7716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7835..7855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7840..8169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8020..8155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8082..8101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8217..8264
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8283..8657
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8787..8908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8824..8843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8878..8897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8957..8973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8957..8973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8970..8992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8981..8997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9005..9011,9012..9029,9030..9035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9050..9071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9188..9205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9359..9947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9439..9458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10118..10909,10910..10978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10741..10760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10979..11083)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11158..11177)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(11349..11598,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtca ccatggccga ctacaaagac gacgatgaca
     2701 agtccgggag tggtgctcta tgggatgtgc ctgctcccaa ggaagtaaaa aagggggaga
     2761 ccacagatgg agtgtacaga gtaatgactc gtagactgct aggttcaaca caagttggag
     2821 tgggagttat gcaagagggg gtctttcaca ctatgtggca cgtcacaaaa ggatccgcgc
     2881 tgagaagcgg tgaagggaga cttgatccat actggggaga tgtcaagcag gatctggtgt
     2941 catactgtgg tccatggaag ctagatgccg cctgggatgg gcacagcgag gtgcagctct
     3001 tggccgtgcc ccccggagag agagcgagga acatccagac tctgcccgga atatttaaga
     3061 caaaggatgg ggacattgga gcggttgcgc tggattaccc agcaggaact tcaggatctc
     3121 caatcctaga caagtgtggg agagtgatag gactttatgg caatggggtc gtgatcaaaa
     3181 acgggagtta tgttagtgcc atcacccaag ggaggaggga ggaagagact cctgttgagt
     3241 gcttcgagcc ctcgatgctg aagaagaagc agctaactgt cttagacttg catcctggag
     3301 ctgggaaaac caggagagtt cttcctgaaa tagtccgtga agccataaaa acaagactcc
     3361 gtactgtgat cttagctcca accagggttg tcgctgctga aatggaggag gcccttagag
     3421 ggcttccagt gcgttatatg acaacagcag tcaatgtcac ccactctgga acagaaatcg
     3481 tcgacttaat gtgccatgcc accttcactt cacgtctact acagccaatc agagtcccca
     3541 actataatct gtatattatg gatgaggccc acttcacaga tccctcaagt atagcagcaa
     3601 gaggatacat ttcaacaagg gttgagatgg gcgaggcggc tgccatcttc atgaccgcca
     3661 cgccaccagg aacccgtgac gcatttccgg actccaactc accaattatg gacaccgaag
     3721 tggaagtccc agagagagcc tggagctcag gctttgattg ggtgacggat cattctggaa
     3781 aaacagtttg gtttgttcca agcgtgagga acggcaatga gatcgcagct tgtctgacaa
     3841 aggctggaaa acgggtcata cagctcagca gaaagacttt tgagacagag ttccagaaaa
     3901 caaaacatca agagtgggac tttgtcgtga caactgacat ttcagagatg ggcgccaact
     3961 ttaaagctga ccgtgtcata gattccagga gatgcctaaa gccggtcata cttgatggcg
     4021 agagagtcat tctggctgga cccatgcctg tcacacatgc cagcgctgcc cagaggaggg
     4081 ggcgcatagg caggaatccc aacaaacctg gagatgagta tctgtatgga ggtgggtgcg
     4141 cagagactga cgaagaccat gcacactggc ttgaagcaag aatgctcctt gacaatattt
     4201 acctccaaga tggcctcata gcctcgctct atcgacctga ggccgacaaa gtagcagcca
     4261 ttgagggaga gttcaagctt aggacggagc aaaggaagac ctttgtggaa ctcatgaaaa
     4321 gaggagatct tcctgtttgg ctggcctatc aggttgcatc tgccggaata acctacacag
     4381 atagaagatg gtgctttgat ggcacgacca acaacaccat aatggaagac agtgtgccgg
     4441 cagaggtgtg gaccagacac ggagagaaaa gagtgctcaa accgaggtgg atggacgcca
     4501 gagtttgttc agatcatgcg gccctgaagt cattcaagga gtttgccgct gggaaaagat
     4561 agcactcgag gatatcgacg cgtgaagctt agcggccgca aattccgccc ctctccctcc
     4621 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     4681 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     4741 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     4801 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     4861 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4921 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     4981 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5041 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     5101 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     5161 ctttgaaaaa cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac
     5221 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5281 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5341 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5401 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5461 ctcgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5521 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5581 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5641 gcgcgctggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5701 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5761 catgacccgc aagcccggtg cctgagttcg cgtctggaac aatcaacctc tggattacaa
     5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6181 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6361 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     6421 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     6481 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     6541 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6601 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     6661 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     6721 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     6781 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     6841 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     6901 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6961 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7021 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7081 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     7141 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7201 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7261 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7321 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7381 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7441 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7501 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7561 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7621 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7681 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7741 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7801 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7861 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7921 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7981 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8041 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8101 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8161 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     8221 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     8281 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     8341 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     8401 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     8461 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     8521 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     8581 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     8641 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     8701 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8761 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     8821 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8881 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     8941 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9001 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9061 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9121 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9181 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9241 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9301 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9361 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9421 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9481 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9541 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9601 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9661 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9721 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9781 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9841 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9901 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9961 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10021 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10081 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10141 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10201 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10261 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10321 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10381 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10441 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10501 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10561 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10621 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10681 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10741 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10801 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10861 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10921 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10981 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11041 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11101 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
    11161 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    11221 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    11281 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    11341 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
    11401 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
    11461 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
    11521 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
    11581 agtacatcaa gtgtatca
//
