LOCUS       sequence-148807-       12459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing NS5 from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12459)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 12459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_NS5_Flag
FEATURES             Location/Qualifiers
     source          1..12459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             complement(3484..3495)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5397..5420
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5469..6041
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5636..5653)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5863..5882
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6044..6646
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6543..6563
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6662..7250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6715..6735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7133..7144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7777..7957
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7983..8000)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7989..8213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8259..8687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8346..8365)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8556..8577
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8696..8716)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8701..9030
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8881..9016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8943..8962
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9078..9125
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9144..9518
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9648..9769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9685..9704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9739..9758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9818..9834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9818..9834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9831..9853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9842..9858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9866..9872,9873..9890,9891..9896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9911..9932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10049..10066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10220..10808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10300..10319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10979..11770,11771..11839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11602..11621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11840..11944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12019..12038)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(12210..12459,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtca ccatgggagg tggaacagga gagaccctgg
     2701 gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac tcctacaaaa
     2761 agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag gacggtgtgg
     2821 caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg gtggagcggg
     2881 gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg ggctggagtt
     2941 actacgtcgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa ggaggccctg
     3001 gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt cttaagagtg
     3061 gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt gacataggtg
     3121 agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc tccatggtgg
     3181 gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc ccatacacca
     3241 gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga ctggtcagag
     3301 tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg aaaagcaaca
     3361 ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac gggcctagga
     3421 ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct gtggtaagct
     3481 gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc cgcagtgagc
     3541 acgcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct taccatggaa
     3601 gctatgaggc ccccacacaa gggtcagcgt cctctctaat aaacggggtt gtcaggctcc
     3661 tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc gacaccacac
     3721 cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca gacccccaag
     3781 aaggcactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag ctaggcaaac
     3841 acaaacggcc acgagtctgc accaaagaag agttcatcaa caaggttcgt agcaatgcag
     3901 cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa gctgtgaacg
     3961 atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga ggagagtgcc
     4021 agagctgtgt gtacaacatg atgggaaaaa gagaaaagaa acaaggggaa tttggaaagg
     4081 ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta gagttcgaag
     4141 cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga ggtggtgttg
     4201 aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgt ataccaggag
     4261 gaaggatgta tgcagatgac actgctggct gggacacccg cattagcagg tttgatctgg
     4321 agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg gcattggcca
     4381 taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct gaaaaaggga
     4441 aaacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa gttgtcactt
     4501 acgctcttaa cacatttacc aacctagtgg tgcaactcat tcggaatatg gaggctgagg
     4561 aagttctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg accaactggt
     4621 tgcagagcaa cggatgggat aggctcaaac gaatggcagt cagtggagat gattgcgttg
     4681 tgaagccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat atgggaaaag
     4741 ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg gaagaagttc
     4801 cgttttgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc attgtggttc
     4861 cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg gcgggatgga
     4921 gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag ctcctttatt
     4981 tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg ccagttgact
     5041 gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg atgaccactg
     5101 aagacatgct tgtggtgtgg aacagagtgt ggattgagga gaacgaccac atggaagaca
     5161 agaccccagt tacgaaatgg acagacattc cctatttggg aaaaagggaa gacttgtggt
     5221 gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt aaaaacacag
     5281 tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac ctatccaccc
     5341 aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtccggg atggccgact
     5401 acaaagacga cgatgacaag tagcactcga ggatatcgac gcgtgaagct tagcggccgc
     5461 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5521 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5581 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5641 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5701 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5761 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5821 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5881 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5941 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6001 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggcca ccgagtacaa
     6061 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     6121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     6181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6301 ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc
     6361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6481 ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc
     6541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagttc gcgtctggaa
     6661 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6841 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6901 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6961 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7021 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7081 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7141 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7201 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     7261 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7321 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7381 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7441 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     7501 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     7561 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     7621 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     7681 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     7741 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     7801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7921 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     7981 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     8041 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     8101 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8161 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     8221 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     8281 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8341 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8401 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8461 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8521 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8581 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8641 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     8701 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8761 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8821 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8881 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8941 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     9001 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     9061 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     9121 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     9181 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     9241 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     9301 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     9361 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     9421 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     9481 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     9541 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9601 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     9661 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9721 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     9781 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9901 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9961 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10021 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10081 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10141 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10621 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10861 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11941 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    12001 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    12061 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    12121 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    12181 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
    12241 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    12301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    12361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    12421 acggtaaact gcccacttgg cagtacatca agtgtatca
//
