LOCUS       sequence-148811-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgTP53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Hong AL, Tseng YY, Cowley GS, Jonas O, Cheah JH, Kynnap BD, Doshi 
            MB, Oh C, Meyer SC, Church AJ, Gill S, Bielski CM, Keskula P, 
            Imamovic A, Howell S, Kryukov GV, Clemons PA, Tsherniak A, Vazquez 
            F, Crompton BD, Shamji AF, Rodriguez-Galindo C, Janeway KA, Roberts 
            CW, Stegmaier K, van Hummelen P, Cima MJ, Langer RS, Garraway LA, 
            Schreiber SL, Root DE, Hahn WC, Boehm JS
  TITLE     Integrated genetic and pharmacologic interrogation of rare cancers.
  JOURNAL   Nat Commun. 2016 Jun 22;7:11987. doi: 10.1038/ncomms11987.
  PUBMED    27329820
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-22"; volume: "7:11987. doi"; pages: " 
            10.1038/ncomms11987"
COMMENT     Sequence Label: sgTP53_3
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..150
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        230..305
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    361..478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        636..847
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcagggctg ttagagagat aattagaatt aatttgactg gtaaacacaa agatattagt
       61 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      121 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      181 atatatcttg tggaaaggac gaaacaccgc cccttgccgt cccaagcaag ttttagagct
      241 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      301 ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa
      361 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      421 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      481 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggtgca
      541 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
      601 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      661 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
      721 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      781 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      841 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
      901 cttgcgtgcc ttgaattact tccccctggc tgcagtacgt gattcttgga
//
