LOCUS       sequence-148816-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shCDK4 targeting 3UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Hong AL, Tseng YY, Cowley GS, Jonas O, Cheah JH, Kynnap BD, Doshi 
            MB, Oh C, Meyer SC, Church AJ, Gill S, Bielski CM, Keskula P, 
            Imamovic A, Howell S, Kryukov GV, Clemons PA, Tsherniak A, Vazquez 
            F, Crompton BD, Shamji AF, Rodriguez-Galindo C, Janeway KA, Roberts 
            CW, Stegmaier K, van Hummelen P, Cima MJ, Langer RS, Garraway LA, 
            Schreiber SL, Root DE, Hahn WC, Boehm JS
  TITLE     Integrated genetic and pharmacologic interrogation of rare cancers.
  JOURNAL   Nat Commun. 2016 Jun 22;7:11987. doi: 10.1038/ncomms11987.
  PUBMED    27329820
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-22"; volume: "7:11987. doi"; pages: " 
            10.1038/ncomms11987"
COMMENT     Sequence Label: TRCN0000279952
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..639)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(123..143)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     620..639
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(724..742)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgtt caggcaccgg gcttgcgggt
       61 catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag
      121 ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc
      181 gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg
      241 gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc
      301 caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg
      361 cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc
      421 cacggcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
      481 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg
      541 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc
      601 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gtaagctccg gtggatcccc
      661 ctggggagag aggtcggtga ttcggtcaac gagggagccg actgccgacg tgcgctccgg
      721 aggcttgcag aatgcggaac accgcgcggg caggaacagg gcccacacta ccgccccaca
      781 ccccgcc
//
