LOCUS       sequence-148819-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2989 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons1. The selective marker is Kl.URA3. 
            Additionally it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2989
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    553..586
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     846..868
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     861..877
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     887..906
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        887..905
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(949..982)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc
       61 tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag
      121 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
      181 tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg
      241 agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct
      301 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat
      361 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg
      421 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa
      481 actataagat gttatcagta tttattatgc atttagaata aattttgtgt cgcccttatt
      541 cgactcacta tagaagttcc tattctctag aaagtatagg aacttcactt caggtctgag
      601 tgcggccgca gatcttgaga aatttgtggg taattagata attgttggga ttccattgtt
      661 gataaaggct ataatattag gtatacagaa tatacactag aagttctcct cgaggatata
      721 ggaatcctca aaatggaatc tatatttcta catactaata ttacgattat tcctcattcc
      781 gtttttgatc aattaatgcc tcagcactaa tgacgtctgt cctgcagggg taacgccagg
      841 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
      901 atagggcgaa ttgggtgcat aggccactag tggatctgat atcacctaat aacttcgtat
      961 agcatacatt atacgaagtt atattaaggg
//
