LOCUS       sequence-148821-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing His10 - SNAPf -9E in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: His10-PS-SNAPf-9E (XSB526)
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     679..698
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        679..697
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             729..751
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             768..797
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             804..827
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
       61 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      121 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      181 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      241 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
      301 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
      361 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
      421 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
      481 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
      541 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
      601 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
      661 atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta
      721 gaaataattt tgtttaactt taagaaggag atatacatat ggaagaacat caccatcacc
      781 atcaccatca ccatcacggt tctctggaag ttctgttcca ggggcccctg ggatccatgg
      841 acaaagactg cgaaatgaag cgcaccaccc tggatagccc tctgggcaag ctggaactgt
      901 ctgggtgcga acagggcctg caccgtatca tcttcctggg caaaggaaca tctgccgccg
      961 acgccgtgga agtgcctgcc cc
//
