LOCUS       sequence-148823-         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing His10 - SNAPf -9R in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: His10-PS-SNAPf-9R (XSB525)
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgagtgcg gccgctcgag tcgacttaac ggcgacgtct gcgtctacga cgacgaccca
      181 gcccaggctt gcccagtctg tggccctcgt gggccagcag ccactctttc acggcgagcc
      241 cgccctcgta gccccccacg tccaggtcgc cctgcaccac ccggtggcag gggatcagaa
      301 tgggcacggg atttccgctc agggcggttt tcacggcggc ggtggcggcg ggattgccgg
      361 ccagggcggc caggtggctg tagctgatga cctctccgaa cttcaccact ttcagcagtt
      421 tccacagcac ctggcgggta aagctctcct gctggaacac tgggtggtgc agggctggca
      481 cagggaactc ctcgatggcc tcaggctggt gaaagtaggc gttgagcca
//
