LOCUS       sequence-148828-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2797 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.URA3. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2797
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..89
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    559..592
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     845..867
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     860..876
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     886..905
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        886..904
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(948..981)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt ttctacaaac
       61 tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg cggttctgat
      121 aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg
      181 ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca ctttgcttga
      241 tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac
      301 gttgcttttt cgattgatga acacctataa ttaaactatt catctattat ttatgatttt
      361 ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc tcgaaaataa
      421 taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat acaaaatata
      481 atacaaacta taagatgtta tcagtattta ttatgcattt agaataaatt ttgtgtcgcc
      541 cttattcgac tcactataga agttcctatt ctctagaaag tataggaact tcacttcagg
      601 tctgagtgcg gccgcagatc tgagaatgtg gattttgatg taattgttgg gattccattt
      661 ttaataaggc aataatatta ggtatgtaga atatactaga agttctcctc aggatttagg
      721 aatccataaa agggaatctg caattctaca caattctata aatattatta tcatcatttt
      781 atattgatca attaatgcct cagcactaat gacgtctgtc ctgcaggggt aacgccaggg
      841 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
      901 tagggcgaat tgggtgcata ggccactagt ggatctgata tcacctaata acttcgtata
      961 gcatacatta tacgaagtta tattaagggt tctcgagagc tcgttttatt taggttctat
//
