LOCUS       sequence-148841-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 with the pLX311 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Hong AL, Tseng YY, Cowley GS, Jonas O, Cheah JH, Kynnap BD, Doshi 
            MB, Oh C, Meyer SC, Church AJ, Gill S, Bielski CM, Keskula P, 
            Imamovic A, Howell S, Kryukov GV, Clemons PA, Tsherniak A, Vazquez 
            F, Crompton BD, Shamji AF, Rodriguez-Galindo C, Janeway KA, Roberts 
            CW, Stegmaier K, van Hummelen P, Cima MJ, Langer RS, Garraway LA, 
            Schreiber SL, Root DE, Hahn WC, Boehm JS
  TITLE     Integrated genetic and pharmacologic interrogation of rare cancers.
  JOURNAL   Nat Commun. 2016 Jun 22;7:11987. doi: 10.1038/ncomms11987.
  PUBMED    27329820
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-22"; volume: "7:11987. doi"; pages: " 
            10.1038/ncomms11987"
COMMENT     Sequence Label: pXPR_BRD111
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..116)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    124..148
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(151..198)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgtt gattgtcgac ttaaccggta
       61 ctagttcatt actacgtaga atcgagaccg aggagagggt tagggatagg cttaccgata
      121 tcaaccactt tgtacaagaa agttgggggt ctttttcttt tttgcctggc cggccttttt
      181 cgtggccgcc ggccttttgt cgcctcccag ctgagacagg tcgatccgtg tctcgtacag
      241 gccggtgatg ctctggtgga tcagggtggc gtccagcacc tctttggtgc tggtgtacct
      301 cttccggtcg atggtggtgt caaagtactt gaaggcggca ggggctccca gattggtcag
      361 ggtaaacagg tggatgatat tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt
      421 gtaggcggac agcactttgt ccagattagc gtcggccagg atcactctct tggagaactc
      481 gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc tgttccacaa acagctgttt
      541 ctgctcatta tcctcggggg agcccttcag cttctcatag tggctggcca ggtacaggaa
      601 gttcacatat ttggagggca gggccagttc gtttcccttc tgcagttcgc cggcagaggc
      661 cagcattctc ttccggccgt tttccagctc gaacagggag tacttaggca gcttgatgat
      721 caggtccttt ttcacttctt tgtagccctt ggcttccaga aagtcgatgg gattcttctc
      781 gaagctgctt ctttccatga tggtgatccc cagcagctct ttcacactct tcagt
//
