LOCUS       sequence-148858-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2992 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons1. The selective marker is Kl.LEU2. 
            Additionally it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2992
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..92
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    562..595
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     855..877
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     870..886
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     896..915
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        896..914
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(958..991)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca
       61 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct
      121 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
      181 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct
      241 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
      301 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
      361 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
      421 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat
      481 ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc
      541 gcccttattc gactcactat agaagttcct attctctaga aagtatagga acttcacttc
      601 aggtctgagt gcggccgcag atcttgagaa atttgtgggt aattagataa ttgttgggat
      661 tccattgttg ataaaggcta taatattagg tatacagaat atacactaga agttctcctc
      721 gaggatatag gaatcctcaa aatggaatct atatttctac atactaatat tacgattatt
      781 cctcattccg tttttgatca attaatgcct cagcactaat gacgtctgtc ctgcaggggt
      841 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      901 cgactcacta tagggcgaat tgggtgcata ggccactagt ggatctgata tcacctaata
      961 acttcgtata gcatacatta tacgaagtta tattaagggt tctcgagagc tcgctgtgaa
     1021 gatcccagca aaggcttaca aagtgttatc
//
