LOCUS       sequence-148866-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2991 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.LEU2. 
            Additionally it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2991
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    555..588
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     847..869
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     862..878
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     888..907
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        888..906
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct acaaactctt
       61 tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt tctgataacg
      121 agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggg
      181 agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt gcttgatata
      241 tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata agatacgttg
      301 ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat gattttttgt
      361 atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga aaataataaa
      421 gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa aatataatac
      481 aaactataag atgttatcag tatttattat gcatttagaa taaattttgt gtcgccctta
      541 ttcgactcac tatagaagtt cctattctct agaaagtata ggaacttcac ttcaggtctg
      601 agtgcggccg cagatcttga gaaatatgtg aattttgaga taattgttgg gattccattg
      661 ttgataaagg ctataatatt aggtatacag aatatactag aagttctcct cgaggattta
      721 ggaatccata aaagggaatc tgcaattcta cacaattcta taaatattat tcatcattcc
      781 gttttatgat caattaatgc ctcagcacta atgacgtctg tcctgcaggg gtaacgccag
      841 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      901 tatagggcga attgggtgca taggccacta gtggatctga tatcacctaa taacttcgta
      961 tagc
//
