LOCUS       sequence-148868-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2991 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.LEU2. 
            Additionally it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2991
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      100..289
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    337..370
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtgtcaaca
       61 acgtatctac caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga
      121 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
      181 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
      241 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc
      301 tgaagcttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
      361 acgaagttat taggtctaga gatcccaata caacagatca cgtgatcttt ttgtaagatg
      421 aagttgaagt agtgttgcac cgtgccaatg caggtggcta ttagattaaa tatgtgattt
      481 gttctattaa gtttcctgta taattacaaa tgaataacga aatgagacaa agaagagaac
      541 caatttttac aagcagccaa gatttccttg acagccttgg cgatagcatc gccaacctca
      601 gtggtagagt tagaaccacc aaggtcaccg gttctgacac ctgcatccaa gacatttcta
      661 acagcttctt caagagccct accttcttca accaaatcca aggataactt caacatcata
      721 gctgcagata agatggtagc aattgggtta accttgtttg ctggtaaatc tggggcagaa
      781 ccatgacatg gttcgtacaa accgaatgcc ttgttagtgt caggtaggga agctagagat
      841 gcagaaggta ataaacccaa agaacctgga ataacagagg cttcatcgga gataatatca
      901 ccaaacatgt tgttggtaat aacaacaccg tttagcttag ttggtgattt aaccaaaatc
      961 atagcagcag agtcgatcaa ttggtgctga acagttaatt
//
