LOCUS       sequence-148874-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the quadruple Holliday 
            junction substrate (QHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Chen SH, Plank JL, Willcox S, Griffith JD, Hsieh TS
  TITLE     Improved methods for creating migratable Holliday junction 
            substrates.
  JOURNAL   Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. 
            Epub 2012 Dec 28.
  PUBMED    23275569
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-03-1"; volume: "41(5):e60. doi"; pages: " 
            10.1093/nar/gks1343. Epub 2012 Dec 28"
COMMENT     Sequence Label: pDHJS4 AS-
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        227..245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    754..787
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(811..827)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(812..828)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(852..871)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(853..871)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(878..895)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(878..894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(887..909)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt aaccctcact
      241 aaagggaaca aaagctggag ctcatatcct acgcccccaa ctgagagaac tcaaaggtta
      301 ccccagttgg ggcactactc tagatcaagt catcaattgt gtgcgcatgc agaacctaat
      361 cttccaatgg ctcggatgcg gccgcacatc aataatctcg tagccaatgt tctcgccctc
      421 gcgatcgcaa tccgtccaga taatcaatcc ctgacagccg cgcacctcgc gttccaaggt
      481 ccttttgata ggctcataat ccgagcccac gcccttttcc accggcgcgt cgaataaaga
      541 gcgcggatcc accgttctcc aatttttgta agacacctgg aatgccagct gcatcatgtg
      601 gccggatacg gaggtcataa ccattttggc attctgaccg cgcacagggg cctcgaaatc
      661 gaagaccttg ttgtacaccg agtagcccgg gccagataat cacgcgtaat cctgacagcc
      721 gcgcacctcg cgttcaaggt cctgaattca gtacgcgccc ggggagccca agggcacgcc
      781 ctggcacccg caccgcgcga tctagcttat cgataccgtc gacctcgagg gggggcccgg
      841 tacccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
      901 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      961 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     1021 tgaatggcga atggaaattg taagcgttaa tattttgtta aaattc
//
