LOCUS       sequence-148876-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2804 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2804
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..88
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    558..591
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     844..866
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     859..875
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     885..904
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        885..903
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(947..980)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccaggcatca aactaagcag aaggcccctg acggatggcc tttttgcgtt tctacaaact
       61 ctttctgtgt tgtaaaacga cggccagtct taagctcggg ccccctgggc ggttctgata
      121 acgagtaatc gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg
      181 gggagtttag ggaaagagca tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat
      241 atatgagaat tatttaacct tataaatgag aaaaaagcaa cgcactttaa ataagatacg
      301 ttgctttttc gattgatgaa cacctataat taaactattc atctattatt tatgattttt
      361 tgtatataca atatttctag tttgttaaag agaattaaga aaataaatct cgaaaataat
      421 aaagggaaaa tcagtttttg atatcaaaat tatacatgtc aacgataata caaaatataa
      481 tacaaactat aagatgttat cagtatttat tatgcattta gaataaattt tgtgtcgccc
      541 ttattcgact cactatagaa gttcctattc tctagaaagt ataggaactt cacttcaggt
      601 ctgagtgcgg ccgcagatct gagaatgtgg attttgatgt aattgttggg attccatttt
      661 taataaggca ataatattag gtatgtagaa tatactagaa gttctcctca ggatttagga
      721 atccataaaa gggaatctgc aattctacac aattctataa atattattat catcatttta
      781 tattgatcaa ttaatgcctc agcactaatg acgtctgtcc tgcaggggta acgccagggt
      841 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
      901 agggcgaatt gggtgcatag gccactagtg gatctgatat cacctaataa cttcgtatag
      961 catacattat acgaagttat attaagggtt c
//
