LOCUS       sequence-148879-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2804 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2804
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      98..287
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    335..368
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tgtcaacaac
       61 gtatctacca acggaatgcg tgcgatcgcg tgcattcatc cgctctaacc gaaaaggaag
      121 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
      181 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
      241 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc gcgtcagctg
      301 aagcttcgta cgctgcaggt cgacaaccct taatataact tcgtataatg tatgctatac
      361 gaagttatta ggtctagaga tcccaataca acagatcacg tgatcttttt gtaagatgaa
      421 gttgaagtag tgttgcaccg tgccaatgca ggtggctatt agattaaata tgtgatttgt
      481 tctattaagt ttcctgtata attacaaatg aataacgaaa tgagacaaag aagagaacca
      541 atttttacaa gcagccaaga tttccttgac agccttggcg atagcatcgc caacctcagt
      601 ggtagagtta gaaccaccaa ggtcaccggt tctgacacct gcatccaaga catttctaac
      661 agcttcttca agagccctac cttcttcaac caaatccaag gataacttca acatcatagc
      721 tgcagataag atggtagcaa ttgggttaac cttgtttgct ggtaaatctg gggcagaacc
      781 atgacatggt tcgtacaaac cgaatgcctt gttagtgtca ggtagggaag ctagagatgc
      841 agaaggtaat aaacccaaag aacctggaat aacagaggct tcatcggaga taatatcacc
      901 aaacatgttg ttggtaataa caacaccgtt tagcttagtt ggtgatttaa ccaaaatcat
      961 agcagcagag tcgatcaatt ggtgctgaac agtt
//
