LOCUS       sequence-148881-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2990 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2990
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..90
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    560..593
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     854..876
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     869..885
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     895..914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        895..913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(957..990)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgccaggcat caaactaagc agaaggcccc tgacggatgg cctttttgcg tttctacaaa
       61 ctctttctgt gttgtaaaac gacggccagt cttaagctcg ggccccctgg gcggttctga
      121 taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg
      181 gggggagttt agggaaagag catttgtcag aatatttaag ggcgcctgtc actttgcttg
      241 atatatgaga attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata
      301 cgttgctttt tcgattgatg aacacctata attaaactat tcatctatta tttatgattt
      361 tttgtatata caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata
      421 ataaagggaa aatcagtttt tgatatcaaa attatacatg tcaacgataa tacaaaatat
      481 aatacaaact ataagatgtt atcagtattt attatgcatt tagaataaat tttgtgtcgc
      541 ccttattcga ctcactatag aagttcctat tctctagaaa gtataggaac ttcacttcag
      601 gtctgagtgc ggccgcagat cttgttgtat tacgggctcg agtaataccg gagtgtcttg
      661 acaatcctaa tataaacagt cttagggaag taaccagttg tcaaaacagt ttatcagatt
      721 aattcacgga atgttactta tcttatatat tatataaaat atgaatcata ctaagtggtg
      781 gaagcgcgga atctgatcaa ttaatgcctc agcactaatg acgtctgtcc tgcaggggta
      841 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      901 gactcactat agggcgaatt gggtgcatag gccactagtg gatctgatat cacctaataa
      961 cttcgtatag catacattat acgaagttat attaagggtt ctcgagagct cgct
//
