LOCUS       sequence-148882-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2990 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2990
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      99..288
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    336..369
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa gtgtcaacaa
       61 cgtatctacc aacggaatgc gtgcgatcgc gtgcattcat ccgctctaac cgaaaaggaa
      121 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
      181 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
      241 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaagat cgcgtcagct
      301 gaagcttcgt acgctgcagg tcgacaaccc ttaatataac ttcgtataat gtatgctata
      361 cgaagttatt aggtctagag atcccaatac aacagatcac gtgatctttt tgtaagatga
      421 agttgaagta gtgttgcacc gtgccaatgc aggtggctat tagattaaat atgtgatttg
      481 ttctattaag tttcctgtat aattacaaat gaataacgaa atgagacaaa gaagagaacc
      541 aatttttaca agcagccaag atttccttga cagccttggc gatagcatcg ccaacctcag
      601 tggtagagtt agaaccacca aggtcaccgg ttctgacacc tgcatccaag acatttctaa
      661 cagcttcttc aagagcccta ccttcttcaa ccaaatccaa ggataacttc aacatcatag
      721 ctgcagataa gatggtagca attgggttaa ccttgtttgc tggtaaatct ggggcagaac
      781 catgacatgg ttcgtacaaa ccgaatgcct tgttagtgtc aggtagggaa gctagagatg
      841 cagaaggtaa taaacccaaa gaacctggaa taacagaggc ttcatcggag ataatatcac
      901 caaacatgtt gttggtaata acaacaccgt ttagcttagt tggtgattta accaaaatca
      961 tagc
//
