LOCUS       sequence-148896-         445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  create protein aggregates in living cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, 
            Wandless TJ
  TITLE     A method to rapidly create protein aggregates in living cells.
  JOURNAL   Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.
  PUBMED    27229621
REFERENCE   2  (bases 1 to 445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-05-27"; volume: "7:11689. doi"; pages: " 
            10.1038/ncomms11689"
COMMENT     Sequence Label: AgDD
FEATURES             Location/Qualifiers
     source          1..445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..340
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     primer_bind     complement(374..402)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctggctggt ctagatattg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac
       61 cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg aagatggaaa
      121 gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga
      181 ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact
      241 gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca
      301 tgccactctc gtcttcgatg tggagcttct aaaaccggaa gaattcagca aaggagaaga
      361 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      421 attttctgtc cgtggagagg gtgaa
//
