LOCUS       sequence-148905-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2803 is a vector for multiple integrations at sites sharing 
            homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally
            it contains a USER cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2803
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      97..286
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    334..367
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatttaataa taaaaatcat aaatcataag aaattcgctt atttagaagt gtcaacaacg
       61 tatctaccaa cggaatgcgt gcgatcgcgt gcattcatcc gctctaaccg aaaaggaagg
      121 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
      181 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
      241 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaagatcg cgtcagctga
      301 agcttcgtac gctgcaggtc gacaaccctt aatataactt cgtataatgt atgctatacg
      361 aagttattag gtctagagat cccaatacaa cagatcacgt gatctttttg taagatgaag
      421 ttgaagtagt gttgcaccgt gccaatgcag gtggctatta gattaaatat gtgatttgtt
      481 ctattaagtt tcctgtataa ttacaaatga ataacgaaat gagacaaaga agagaaccaa
      541 tttttacaag cagccaagat ttccttgaca gccttggcga tagcatcgcc aacctcagtg
      601 gtagagttag aaccaccaag gtcaccggtt ctgacacctg catccaagac atttctaaca
      661 gcttcttcaa gagccctacc ttcttcaacc aaatccaagg ataacttcaa catcatagct
      721 gcagataaga tggtagcaat tgggttaacc ttgtttgctg gtaaatctgg ggcagaacca
      781 tgacatggtt cgtacaaacc gaatgccttg ttagtgtcag gtagggaagc tagagatgca
      841 gaaggtaata aacccaaaga acctggaata acagaggctt catcggagat aatatcacca
      901 aacatgttgt tggtaataac aacaccgttt agcttagttg gtgatttaac caaaatcata
      961 gcagcagagt cgatcaattg gtgctgaaca gttaattgtg ggaactcagt cttgatggtt
     1021 tcttcaacag tcttt
//
