LOCUS       sequence-148906-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for LbCpf1 guide RNA (need to clone in 
            spacer into BsmBI sites).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, 
            McCaw ZR, Aryee MJ, Joung JK
  TITLE     Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
            cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
  PUBMED    27347757
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620."
COMMENT     Sequence Label: BPK3082
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        399..647
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     399..419
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     570..589
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 cccgaaaagt gccacctgac gtcgctagct gtacaaaaaa gcaggcttta aaggaaccaa
      361 ttcagtcgac tggatccggt accaaggtcg ggcaggaaga gggcctattt cccatgattc
      421 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
      481 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
      541 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
      601 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgaa tttctactaa
      661 gtgtagatgg agacgattaa tgcgtctcct ttttttaagc ttgggccgct cgaggtacct
      721 ctctacatat gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      841 aagtcaga
//
