LOCUS       sequence-148912-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for AsCpf1-NLS-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, 
            McCaw ZR, Aryee MJ, Joung JK
  TITLE     Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
            cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
  PUBMED    27347757
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620."
COMMENT     Sequence Label: SQT1659
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(173..253)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(260..307)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      579..588
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg angtccccat
       61 aatttttggc agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac
      121 cagccaccac cttctgatag gcagcctgca cctgaggagt gcggccgctt taggcatagt
      181 cggggacatc atatgggtat gcataatcag gcacgtcgta gggataagcg taatctggaa
      241 catcgtatgg gtaggatccc tttttctttt ttgcctggcc ggcctttttc gtggccgccg
      301 gccttttgtt gcgcagctcc tggatgtagg ccagccagtc ctgattggag atgccgttct
      361 gcagcttcag atccttgctc tccttcaggt gattcagcag cagctggccc ttcagggcga
      421 tgtggtaggc gccattggca tcggcgtcca tgggccactc tgggttctga aaccgggagt
      481 cgaagcacac gccattcaga tcgcgcacgg ggctgttgat atagtcctcg cctgtggcgg
      541 cattggagtt ccgcatctgc agcacgctgc ggatcagggc caccatggtg tcgatggcgt
      601 gagaatcgtc attctccagc agctttggca ggatgttgga gccatccctg aacacgatgc
      661 ccttctcctc cagcagggcg atcagctcgt tggcaggata caggtcccgg tatctgccgg
      721 tgaatctgtg attctcgatc actggcacga ttctcttgcc ggcgatgaaa ggggtgccct
      781 tggcgtcaaa ctgtgtctcg ttcttctcga acacgatatc ccatgcaggc ataaagccgg
      841 g
//
