LOCUS       sequence-148914-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2795 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons1. The selective marker is Kl.URA3. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2795
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..89
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    559..592
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     852..874
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     867..883
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     893..912
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        893..911
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(955..988)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccaggcatc aaactaagca gaaggcccct gacggatggc ctttttgcgt ttctacaaac
       61 tctttctgtg ttgtaaaacg acggccagtc ttaagctcgg gccccctggg cggttctgat
      121 aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg
      181 ggggagttta gggaaagagc atttgtcaga atatttaagg gcgcctgtca ctttgcttga
      241 tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac
      301 gttgcttttt cgattgatga acacctataa ttaaactatt catctattat ttatgatttt
      361 ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc tcgaaaataa
      421 taaagggaaa atcagttttt gatatcaaaa ttatacatgt caacgataat acaaaatata
      481 atacaaacta taagatgtta tcagtattta ttatgcattt agaataaatt ttgtgtcgcc
      541 cttattcgac tcactataga agttcctatt ctctagaaag tataggaact tcacttcagg
      601 tctgagtgcg gccgcagatc ttgagaaatt tgtgggtaat tagataattg ttgggattcc
      661 attgttgata aaggctataa tattaggtat acagaatata cactagaagt tctcctcgag
      721 gatataggaa tcctcaaaat ggaatctata tttctacata ctaatattac gattattcct
      781 cattccgttt ttgatcaatt aatgcctcag cactaatgac gtctgtcctg caggggtaac
      841 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
      901 ctcactatag ggcgaattgg gtgcataggc cactagtgga tctgatatca cctaataact
      961 tcgtatagca tacattatac gaagttatat taagggttct cgagagctcg ttttatttag
     1021 gttctatcg
//
