LOCUS       sequence-148917-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for LbCpf1-NLS-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, 
            McCaw ZR, Aryee MJ, Joung JK
  TITLE     Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
            cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
  PUBMED    27347757
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620."
COMMENT     Sequence Label: SQT1665
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(177..257)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(264..311)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
       61 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
      121 acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgcggcc gctttaggca
      181 tagtcgggga catcatatgg gtatgcataa tcaggcacgt cgtagggata agcgtaatct
      241 ggaacatcgt atgggtagga tccctttttc ttttttgcct ggccggcctt tttcgtggcc
      301 gccggccttt tgtgcttcac gctggtctgg gcgtactcca gccactcctt gttagagatg
      361 gcgatcttca ccttatccag cttctcgtcc tcggccttct tgaactggcc gatggcccac
      421 agcacctttc tggcgatgtt ataggcgcca ttggcgtcgg cgttctttgg caggatggca
      481 ttctcctggg cctcatagtt ccggctatcg tagaagatgc cgtcggagtt cttcacaggg
      541 ctgatcagaa aatccacgtc ggtgcggcct gtgatgctgt tccgcatctg cagcatcagg
      601 ctcatcaggg ccataaagct agagtagaag gccttgtcgg actgctcgca cagcagggct
      661 ctgatatcgc cctgctgata attgatgccg tacttgttga acagctcctt ataggcgctg
      721 gtcaggcaca cctcctccca gtcgaacacg ttgttcttct taggattccg gaagattctg
      781 atccggttgc cgta
//
