LOCUS       sequence-148929-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2794 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.URA3. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2794
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..92
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    562..595
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     854..876
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     869..885
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     895..914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        895..913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca
       61 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct
      121 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
      181 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct
      241 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
      301 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
      361 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
      421 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat
      481 ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc
      541 gcccttattc gactcactat agaagttcct attctctaga aagtatagga acttcacttc
      601 aggtctgagt gcggccgcag atcttgagaa atatgtgaat tttgagataa ttgttgggat
      661 tccattgttg ataaaggcta taatattagg tatacagaat atactagaag ttctcctcga
      721 ggatttagga atccataaaa gggaatctgc aattctacac aattctataa atattattca
      781 tcattccgtt ttatgatcaa ttaatgcctc agcactaatg acgtctgtcc tgcaggggta
      841 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      901 gactcactat agggcgaatt gggtgcatag gccactagtg gatctgatat cacctaataa
      961 cttcgtatag catacatt
//
