LOCUS       sequence-148931-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2794 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.URA3. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2794
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      47..236
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    284..317
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attacacatg gcatggatga gctctacaaa tgaatcgcgt gcattcatcc gctctaaccg
       61 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt
      121 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt
      181 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaagatcg
      241 cgtcagctga agcttcgtac gctgcaggtc gacaaccctt aatataactt cgtataatgt
      301 atgctatacg aagttattag gtctagagat cccaatacaa cagatcacgt gatctttttg
      361 taagatgaag ttgaagtagt gttgcaccgt gccaatgcag gtggctatta gattaaatat
      421 gtgatttgtt ctattaagtt tcctgtataa ttacaaatga ataacgaaat gagacaaaga
      481 agagaaccaa tttttacaag catggggagc gctgattctc ttttggtacg cttcccatcc
      541 agcatttctg tatctttcac cttcaacctt aggatctcta cccttggcga aaagtcctct
      601 gccaacaatg atgatatctg atccaccact tacaacttcg tcgacggttc tgtactgctg
      661 acccaatgca tcgcctttgt cgtctaaacc tacacctggg gtcatgatta gccaatcaaa
      721 cccttcttct cttcctccca tatcgttctg agcaatgaac ccaataacga aatctttatc
      781 actctttgca atatcaacgg tacccttagt atattcaccg tgtgctagag aacccttgga
      841 agataattca gcaagcatca ataatcccct tggttctttg gtgacctctt gcgcaccttg
      901 tttcaagcca gcaacaatac cagcaccagt aaccccgtgg gcgttggtga tatcagacca
      961 ttctgcgata cggtaaacgc ccgatgtat
//
