LOCUS       sequence-148933-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid template that encodes the photoconvertible fluorescent 
            protein mEos3.2 next to chloramphenicol resistance gene (cat). This 
            template was used to copy out mEos3.2-cat for genetic tagging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Chen TY, Santiago AG, Jung W, Krzeminski L, Yang F, Martell DJ, 
            Helmann JD, Chen P
  TITLE     Concentration- and chromosome-organization-dependent regulator 
            unbinding from DNA for transcription regulation in living cells.
  JOURNAL   Nat Commun. 2015 Jul 6;6:7445. doi: 10.1038/ncomms8445.
  PUBMED    26145755
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-6"; volume: "6:7445. doi"; pages: " 
            10.1038/ncomms8445"
COMMENT     Sequence Label: pUCmEos3.2:cat
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..708
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="SAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNARN
                     DITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     protein_bind    complement(735..782)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gctggctccg ctgctggttc tggcgaattc agtgcgatta agccagacat gaagatcaaa
       61 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      121 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
      181 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccaaatat
      241 ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      301 agcttgactt tcgaagacgg gggcatttgc aacgccagaa acgacataac aatggaaggg
      361 gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt
      421 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      481 gtgctgacgg gtgatattga gatggctttg ttgcttgaag gaaatgccca ttaccgatgt
      541 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg cgcccacttt
      601 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      661 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgataagt cgacgtgtag
      721 gctggagctg cttcgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
      781 tcatttaaat ggcgcgcctt acgccccgcc ctgccactca tcgcagtact gttgtaattc
      841 attaagcatt
//
