LOCUS       sequence-148934-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid template that encodes the photoconvertible fluorescent 
            protein mEos3.2 next to chloramphenicol resistance gene (cat). This 
            template was used to copy out mEos3.2-cat for genetic tagging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Chen TY, Santiago AG, Jung W, Krzeminski L, Yang F, Martell DJ, 
            Helmann JD, Chen P
  TITLE     Concentration- and chromosome-organization-dependent regulator 
            unbinding from DNA for transcription regulation in living cells.
  JOURNAL   Nat Commun. 2015 Jul 6;6:7445. doi: 10.1038/ncomms8445.
  PUBMED    26145755
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-6"; volume: "6:7445. doi"; pages: " 
            10.1038/ncomms8445"
COMMENT     Sequence Label: pUCmEos3.2:cat
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..52
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        158..260
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(327..346)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctccttagtt cctattccga agttcctatt ctctagaaag tataggaact tcggcgcgcc
       61 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
      121 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
      181 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagttg tcgagatttt
      241 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      301 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      361 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      421 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattac
      481 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      541 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      601 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      661 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      721 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      781 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      841 ccgtttgtga
//
