LOCUS       sequence-148937-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2792 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.URA3. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2792
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      48..237
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    285..318
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gattacacat ggcatggatg agctctacaa atgaatcgcg tgcattcatc cgctctaacc
       61 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
      121 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
      181 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc
      241 gcgtcagctg aagcttcgta cgctgcaggt cgacaaccct taatataact tcgtataatg
      301 tatgctatac gaagttatta ggtctagaga tcccaataca acagatcacg tgatcttttt
      361 gtaagatgaa gttgaagtag tgttgcaccg tgccaatgca ggtggctatt agattaaata
      421 tgtgatttgt tctattaagt ttcctgtata attacaaatg aataacgaaa tgagacaaag
      481 aagagaacca atttttacaa gcatggggag cgctgattct cttttggtac gcttcccatc
      541 cagcatttct gtatctttca ccttcaacct taggatctct acccttggcg aaaagtcctc
      601 tgccaacaat gatgatatct gatccaccac ttacaacttc gtcgacggtt ctgtactgct
      661 gacccaatgc atcgcctttg tcgtctaaac ctacacctgg ggtcatgatt agccaatcaa
      721 acccttcttc tcttcctccc atatcgttct gagcaatgaa cccaataacg aaatctttat
      781 cactctttgc aatatcaacg gtacccttag tatattcacc gtgtgctaga gaacccttgg
      841 aagataattc agcaagcatc aataatcccc ttggttcttt ggtgacctct tgcgcacctt
      901 gtttcaagcc agcaacaata ccagcaccag taaccccgtg ggcgttggtg atatcagacc
      961 attctgcgat acggtaaacg cccgatgtat attg
//
