LOCUS       sequence-148941-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating LexA::GADfluw lines from MiMIC lines with inserts into 
            coding introns in Phase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..28
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10..28
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    68..137
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 ccagcgcgca attaaccctc actaaaggga acaaaagctg ggtaccatgc atgatgtagg
       61 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
      121 actccacctc acccatctgg tccatcatga tggtcgacaa gcttagaagt tcaaatgggc
      181 tttcatattt acttttttct ctgagtgggt aaggtcattg tggggtctta agggctaaag
      241 ggtagtgatg atgaaggcat ttgatgccat tgttcaataa cttacctccg gaacctccgc
      301 ttccaccgct acctccggat ctttaattaa aaacgagttt ttaagcaaac tcactccctg
      361 acaataaaaa cgaattatct ttaaataaat atattctatt tattaaccaa gtagctaata
      421 tagctaaaat caatttgttg ctaacttaaa actaacataa ttatgtatta gttatttaca
      481 ttcttttggt tggttacaac caaataaata atatcacttt taaaaactta agccagaact
      541 gaataatttc tatttggctt tagtcgacgg tatcgataag cttctgaata agccctcgta
      601 atatattttc atgaagaatt taggtccaaa aaaaagatgg gcattaattc tagtcattta
      661 aaaaattcta tagatcagag gttacatggc caagattgaa acttagagga gtatagttac
      721 ataaaagaag gcaaaacgat gtataaatga aagaaattga gatggtgcac gatgcacagt
      781 tgaagtgaac ttgcggggtt tttcagtatc tacgattcat tttattaatt aacctaggct
      841 actccttctt tgggttcggt ggagtatcct catcatcaa
//
