LOCUS       sequence-148946-         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  self-killing gRNA plasmid pMAZ-SK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 238 to 272)
  AUTHORS   Ronda C, Pedersen LE, Sommer MO, Nielsen AT
  TITLE     CRMAGE: CRISPR Optimized MAGE Recombineering.
  JOURNAL   Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452.
  PUBMED    26797514
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-01-22"; volume: "6:19452. doi"; pages: " 
            10.1038/srep19452"
COMMENT     Sequence Label: pMAZ-SK
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(159..237)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        complement(238..272)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     repeat_region   409..444
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   475..510
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   541..576
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
       61 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgcaca ccgactagcg
      121 aaaaaacccc gccgaagcgg ggttttttgc gaaaaaaagc accgactcgg tgccactttt
      181 tcaagttgat aacggactag ccttatttta acttgctatg ctgttttgaa tggttccgct
      241 agcactgtac ctaggactga gctagccgtc aaaagcagcg tatataccat ggccacaatt
      301 cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct
      361 gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtgt tttagagcta
      421 tgctgttttg aatggtccca aaacccgttc cgtgtagaca gttcgctcca agctgtttta
      481 gagctatgct gttttgaatg gtcccaaaac gccatgtttc agaaacaact ctggcgcatc
      541 gttttagagc tatgctgttt tgaatggtcc caaaacaaaa aaaaaccccg cccc
//
