LOCUS       sequence-148947-         383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  self-killing gRNA plasmid pMAZ-SK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Ronda C, Pedersen LE, Sommer MO, Nielsen AT
  TITLE     CRMAGE: CRISPR Optimized MAGE Recombineering.
  JOURNAL   Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452.
  PUBMED    26797514
REFERENCE   2  (bases 1 to 383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-01-22"; volume: "6:19452. doi"; pages: " 
            10.1038/srep19452"
COMMENT     Sequence Label: pMAZ-SK
FEATURES             Location/Qualifiers
     source          1..383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(274..321)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     317..335
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      121 cgaaaagtgc cacttgcgga gacccggtcg tcagcttgtc gtcggttcag ggcagggtcg
      181 ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt
      241 gaccgtgtgc ttctcaaatg cctgaggttt cagcaaaaaa cccctcaaga cccgtttaga
      301 ggccccaagg ggttatgcta gttattgctc agcggtggca gcagcctagg ataccggtag
      361 gctgcataaa aaagcaccga ctc
//
