LOCUS       sequence-148948-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2791 is a vector for multiple integrations at sites sharing 
            homology with Ty4Cons. The selective marker is Kl.URA3. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2791
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..789
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(106..134)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      806..995
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaattacaa
       61 atctgtcata aaacaatgag caaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagaggaga gggtgaaggt
      181 gatgctacaa tcggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc ccgttatccg
      301 gatcacatga aaaggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 cgcactatat ctttcaaaga tgacgggaaa tacaagacgc gtgctgtagt caagtttgaa
      421 ggtgataccc ttgttaatcg tatcgagtta aagggtactg attttaaaga agatggaaac
      481 attctcggac acaaactcga gtacaacttt aactcacaca atgtatacat cacggcagac
      541 aaacaaaaga atggaatcaa agctaacttc acagttcgcc acaacgttga agatggttcc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc gacacaaact gtcctttcga aagatcccaa cgaaaagcgt
      721 gaccacatgg tccttcatga gtacgtaaat gctgctggga ttacacatgg catggatgag
      781 ctctacaaat gaatcgcgtg cattcatccg ctctaaccga aaaggaagga gttagacaac
      841 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
      901 tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga
      961 aaaccttgct tgagaaggtt ttgggacgct cgaagatcgc gtcagctgaa gcttc
//
