LOCUS       sequence-148951-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2791 is a vector for multiple integrations at sites sharing 
            homology with Ty4Cons. The selective marker is Kl.URA3. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2791
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      54..243
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    291..324
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgctgggatt acacatggca tggatgagct ctacaaatga atcgcgtgca ttcatccgct
       61 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
      121 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
      181 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
      241 aagatcgcgt cagctgaagc ttcgtacgct gcaggtcgac aacccttaat ataacttcgt
      301 ataatgtatg ctatacgaag ttattaggtc tagagatccc aatacaacag atcacgtgat
      361 ctttttgtaa gatgaagttg aagtagtgtt gcaccgtgcc aatgcaggtg gctattagat
      421 taaatatgtg atttgttcta ttaagtttcc tgtataatta caaatgaata acgaaatgag
      481 acaaagaaga gaaccaattt ttacaagcat ggggagcgct gattctcttt tggtacgctt
      541 cccatccagc atttctgtat ctttcacctt caaccttagg atctctaccc ttggcgaaaa
      601 gtcctctgcc aacaatgatg atatctgatc caccacttac aacttcgtcg acggttctgt
      661 actgctgacc caatgcatcg cctttgtcgt ctaaacctac acctggggtc atgattagcc
      721 aatcaaaccc ttcttctctt cctcccatat cgttctgagc aatgaaccca ataacgaaat
      781 ctttatcact ctttgcaata tcaacggtac ccttagtata ttcaccgtgt gctagagaac
      841 ccttggaaga taattcagca agcatcaata atccccttgg ttctttggtg acctcttgcg
      901 caccttgttt caagccagca acaataccag caccagtaac cccgtgggcg ttggtgatat
      961 cagaccattc tgcgatacgg taaacgcccg atgtata
//
