LOCUS       sequence-148954-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2802 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons1. The selective marker is Kl.LEU2. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2802
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..87
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    557..590
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     850..872
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     865..881
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     891..910
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        891..909
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caggcatcaa actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc
       61 tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttctgataa
      121 cgagtaatcg ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg
      181 ggagtttagg gaaagagcat ttgtcagaat atttaagggc gcctgtcact ttgcttgata
      241 tatgagaatt atttaacctt ataaatgaga aaaaagcaac gcactttaaa taagatacgt
      301 tgctttttcg attgatgaac acctataatt aaactattca tctattattt atgatttttt
      361 gtatatacaa tatttctagt ttgttaaaga gaattaagaa aataaatctc gaaaataata
      421 aagggaaaat cagtttttga tatcaaaatt atacatgtca acgataatac aaaatataat
      481 acaaactata agatgttatc agtatttatt atgcatttag aataaatttt gtgtcgccct
      541 tattcgactc actatagaag ttcctattct ctagaaagta taggaacttc acttcaggtc
      601 tgagtgcggc cgcagatctt gagaaatttg tgggtaatta gataattgtt gggattccat
      661 tgttgataaa ggctataata ttaggtatac agaatataca ctagaagttc tcctcgagga
      721 tataggaatc ctcaaaatgg aatctatatt tctacatact aatattacga ttattcctca
      781 ttccgttttt gatcaattaa tgcctcagca ctaatgacgt ctgtcctgca ggggtaacgc
      841 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
      901 cactataggg cgaattgggt gcataggcca ctagtggatc tgatatcacc taataacttc
      961 gtatagcata cattatac
//
