LOCUS       sequence-148955-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the quadruple Holliday 
            junction substrate (QHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Chen SH, Plank JL, Willcox S, Griffith JD, Hsieh TS
  TITLE     Improved methods for creating migratable Holliday junction 
            substrates.
  JOURNAL   Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. 
            Epub 2012 Dec 28.
  PUBMED    23275569
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-03-1"; volume: "41(5):e60. doi"; pages: " 
            10.1093/nar/gks1343. Epub 2012 Dec 28"
COMMENT     Sequence Label: pDHJS4 BS-
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     236..258
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     250..267
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     251..267
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     274..293
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        274..292
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     317..333
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     318..334
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    358..391
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(663..682)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(848..866)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(887..903)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(887..903)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(900..922)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    911..927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(935..941,942..959,960..965))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    980..1001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
       61 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca
      121 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      181 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      241 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
      301 ggtaccgggc cccccctcga ggtcgacggt atcgataagc tagatcgcgc ggtgcgggtg
      361 ccagggcgtg cccttgggct ccccgggcgc gtactgaatt caggaccttg aacgcgaggt
      421 gcgcggctgt caggattacg cgtgattatc tggcccgggg ctatatgcgt tgatgcaatt
      481 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
      541 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
      601 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      661 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg gcggccgcat
      721 ccgagccatt ggaagattag gttctgcatg cgcacacaat tgatgacttg atctagagta
      781 gtgccccaac tggggtaacc tttgagttct ctcagttggg ggcgtaggtg agctccagct
      841 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
      901 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      961 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1021 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caac
//
