LOCUS       sequence-148957-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  One of four plasmids needed to create the mismatched quadruple 
            Holliday junction substrate (MM-QHJS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Chen SH, Plank JL, Willcox S, Griffith JD, Hsieh TS
  TITLE     Improved methods for creating migratable Holliday junction 
            substrates.
  JOURNAL   Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. 
            Epub 2012 Dec 28.
  PUBMED    23275569
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-03-1"; volume: "41(5):e60. doi"; pages: " 
            10.1093/nar/gks1343. Epub 2012 Dec 28"
COMMENT     Sequence Label: pDHJS4 AN+
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     187..209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     201..218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     202..218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     225..244
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        225..243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     268..284
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     269..285
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    309..342
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        complement(851..869)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(890..906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(890..906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(903..925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    914..930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(938..944,945..962,963..968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    983..1004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      181 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
      241 agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag ctagatcgcg
      301 cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactgaat tcaggacctt
      361 gaacgcgagg tgcgcggctg tcaggattac gcgtgattat ctggcccggg ctactcggtg
      421 tacaacaagg tcttcgattt cgaggcccct gtgcgcggtc agaatgccaa aatggttatg
      481 acctccgtat ccggccacat gatgcagctg gcattccagg tgtcttacaa aaattggaga
      541 acggtggatc cgcgctcttt attcgacgcg ccggtggaaa agggcgtggg ctcggattat
      601 gagcctatca aaaggacctt ggaacgcgag gtgcgcggct gtcagggatt gattatctgg
      661 acggattgcg atcgcgaggg cgagaacatt ggctacgaga ttattgatgt gcggccgcat
      721 ccgagccatt ggaagattag gttctgctag cgcacacaat tgatgacttg atctagagta
      781 gtgccccaac tggggtaacc tttgagttct ctcagttggg ggcgtaggat atgagctcca
      841 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
      901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1021 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
//
