LOCUS       sequence-148959-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2802 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons1. The selective marker is Kl.LEU2. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2802
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..792
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(109..137)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      809..998
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttaatta
       61 caaatctgtc ataaaacaat gagcaaagga gaagaacttt tcactggagt tgtcccaatt
      121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagagg agagggtgaa
      181 ggtgatgcta caatcggaaa actcaccctt aaatttattt gcactactgg aaaactacct
      241 gttccatggc caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat
      301 ccggatcaca tgaaaaggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      361 gaacgcacta tatctttcaa agatgacggg aaatacaaga cgcgtgctgt agtcaagttt
      421 gaaggtgata cccttgttaa tcgtatcgag ttaaagggta ctgattttaa agaagatgga
      481 aacattctcg gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca
      541 gacaaacaaa agaatggaat caaagctaac ttcacagttc gccacaacgt tgaagatggt
      601 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      661 ttaccagaca accattacct gtcgacacaa actgtccttt cgaaagatcc caacgaaaag
      721 cgtgaccaca tggtccttca tgagtacgta aatgctgctg ggattacaca tggcatggat
      781 gagctctaca aatgaatcgc gtgcattcat ccgctctaac cgaaaaggaa ggagttagac
      841 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
      901 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
      961 tgaaaacctt gcttgagaag gttttgggac gctcgaagat cgcgtcagct ga
//
