LOCUS       sequence-148960-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2802 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons1. The selective marker is Kl.LEU2. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2802
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      55..244
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    292..325
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgctgggat tacacatggc atggatgagc tctacaaatg aatcgcgtgc attcatccgc
       61 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
      121 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
      181 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
      241 gaagatcgcg tcagctgaag cttcgtacgc tgcaggtcga caacccttaa tataacttcg
      301 tataatgtat gctatacgaa gttattaggt ctagagatcc caatacaaca gatcacgtga
      361 tctttttgta agatgaagtt gaagtagtgt tgcaccgtgc caatgcaggt ggctattaga
      421 ttaaatatgt gatttgttct attaagtttc ctgtataatt acaaatgaat aacgaaatga
      481 gacaaagaag agaaccaatt tttacaagca gccaagattt ccttgacagc cttggcgata
      541 gcatcgccaa cctcagtggt agagttagaa ccaccaaggt caccggttct gacacctgca
      601 tccaagacat ttctaacagc ttcttcaaga gccctacctt cttcaaccaa atccaaggat
      661 aacttcaaca tcatagctgc agataagatg gtagcaattg ggttaacctt gtttgctggt
      721 aaatctgggg cagaaccatg acatggttcg tacaaaccga atgccttgtt agtgtcaggt
      781 agggaagcta gagatgcaga aggtaataaa cccaaagaac ctggaataac agaggcttca
      841 tcggagataa tatcaccaaa catgttgttg gtaataacaa caccgtttag cttagttggt
      901 gatttaacca aaatcatagc agcagagtcg atcaattggt gctgaacagt taattgtggg
      961 aactcagtct tgatggtttc
//
