LOCUS       sequence-148967-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2801 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.LEU2. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2801
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     77..93
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    563..596
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     855..877
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     870..886
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     896..915
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        896..914
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(958..991)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aactgccagg catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac
       61 aaactctttc tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc
      121 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
      181 atggggggag tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc
      241 ttgatatatg agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag
      301 atacgttgct ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga
      361 ttttttgtat atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa
      421 ataataaagg gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa
      481 tataatacaa actataagat gttatcagta tttattatgc atttagaata aattttgtgt
      541 cgcccttatt cgactcacta tagaagttcc tattctctag aaagtatagg aacttcactt
      601 caggtctgag tgcggccgca gatcttgaga aatatgtgaa ttttgagata attgttggga
      661 ttccattgtt gataaaggct ataatattag gtatacagaa tatactagaa gttctcctcg
      721 aggatttagg aatccataaa agggaatctg caattctaca caattctata aatattattc
      781 atcattccgt tttatgatca attaatgcct cagcactaat gacgtctgtc ctgcaggggt
      841 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      901 cgactcacta tagggcgaat tgggtgcata ggccactagt ggatctgata tcacctaata
      961 acttcgtata gcatacatta tacgaagtta tattaaggg
//
