LOCUS       sequence-148968-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2801 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.LEU2. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2801
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..795
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(112..140)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      812..1001
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttaa
       61 ttacaaatct gtcataaaac aatgagcaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag aggagagggt
      181 gaaggtgatg ctacaatcgg aaaactcacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ctgacctatg gtgttcaatg cttttcccgt
      301 tatccggatc acatgaaaag gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaacgca ctatatcttt caaagatgac gggaaataca agacgcgtgc tgtagtcaag
      421 tttgaaggtg atacccttgt taatcgtatc gagttaaagg gtactgattt taaagaagat
      481 ggaaacattc tcggacacaa actcgagtac aactttaact cacacaatgt atacatcacg
      541 gcagacaaac aaaagaatgg aatcaaagct aacttcacag ttcgccacaa cgttgaagat
      601 ggttccgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtcgaca caaactgtcc tttcgaaaga tcccaacgaa
      721 aagcgtgacc acatggtcct tcatgagtac gtaaatgctg ctgggattac acatggcatg
      781 gatgagctct acaaatgaat cgcgtgcatt catccgctct aaccgaaaag gaaggagtta
      841 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt
      901 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta
      961 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa gatcgcgtca gctgaagctt
     1021 cgtacgctgc aggtcgacaa ccctt
//
