LOCUS       sequence-148969-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2801 is a vector for multiple integrations at sites sharing 
            homology with Ty1Cons2. The selective marker is Kl.LEU2. 
            Additionally it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2801
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      49..238
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    286..319
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggattacaca tggcatggat gagctctaca aatgaatcgc gtgcattcat ccgctctaac
       61 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat
      121 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt
      181 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaagat
      241 cgcgtcagct gaagcttcgt acgctgcagg tcgacaaccc ttaatataac ttcgtataat
      301 gtatgctata cgaagttatt aggtctagag atcccaatac aacagatcac gtgatctttt
      361 tgtaagatga agttgaagta gtgttgcacc gtgccaatgc aggtggctat tagattaaat
      421 atgtgatttg ttctattaag tttcctgtat aattacaaat gaataacgaa atgagacaaa
      481 gaagagaacc aatttttaca agcagccaag atttccttga cagccttggc gatagcatcg
      541 ccaacctcag tggtagagtt agaaccacca aggtcaccgg ttctgacacc tgcatccaag
      601 acatttctaa cagcttcttc aagagcccta ccttcttcaa ccaaatccaa ggataacttc
      661 aacatcatag ctgcagataa gatggtagca attgggttaa ccttgtttgc tggtaaatct
      721 ggggcagaac catgacatgg ttcgtacaaa ccgaatgcct tgttagtgtc aggtagggaa
      781 gctagagatg cagaaggtaa taaacccaaa gaacctggaa taacagaggc ttcatcggag
      841 ataatatcac caaacatgtt gttggtaata acaacaccgt ttagcttagt tggtgattta
      901 accaaaatca tagcagcaga gtcgatcaat tggtgctgaa cagttaattg tgggaactca
      961 gtcttgatgg tttcttcaac agtctttctc c
//
