LOCUS       sequence-148974-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2800 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2800
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..82
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    552..585
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     838..860
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     853..869
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     879..898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        879..897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(941..974)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct
       61 gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt
      121 aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt
      181 ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga
      241 gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt
      301 tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata
      361 tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg
      421 aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa
      481 ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttattc
      541 gactcactat agaagttcct attctctaga aagtatagga acttcacttc aggtctgagt
      601 gcggccgcag atctgagaat gtggattttg atgtaattgt tgggattcca tttttaataa
      661 ggcaataata ttaggtatgt agaatatact agaagttctc ctcaggattt aggaatccat
      721 aaaagggaat ctgcaattct acacaattct ataaatatta ttatcatcat tttatattga
      781 tcaattaatg cctcagcact aatgacgtct gtcctgcagg ggtaacgcca gggttttccc
      841 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
      901 aattgggtgc ataggccact agtggatctg atatcaccta ataacttcgt atagcataca
      961 ttatacgaag ttatattaag ggttctcgag agctcgctgt gaagatccca
//
