LOCUS       sequence-148984-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2800 is a vector for multiple integrations at sites sharing 
            homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2800
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      56..245
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    293..326
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctgctggga ttacacatgg catggatgag ctctacaaat gaatcgcgtg cattcatccg
       61 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
      121 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
      181 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
      241 cgaagatcgc gtcagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc
      301 gtataatgta tgctatacga agttattagg tctagagatc ccaatacaac agatcacgtg
      361 atctttttgt aagatgaagt tgaagtagtg ttgcaccgtg ccaatgcagg tggctattag
      421 attaaatatg tgatttgttc tattaagttt cctgtataat tacaaatgaa taacgaaatg
      481 agacaaagaa gagaaccaat ttttacaagc agccaagatt tccttgacag ccttggcgat
      541 agcatcgcca acctcagtgg tagagttaga accaccaagg tcaccggttc tgacacctgc
      601 atccaagaca tttctaacag cttcttcaag agccctacct tcttcaacca aatccaagga
      661 taacttcaac atcatagctg cagataagat ggtagcaatt gggttaacct tgtttgctgg
      721 taaatctggg gcagaaccat gacatggttc gtacaaaccg aatgccttgt tagtgtcagg
      781 tagggaagct agagatgcag aaggtaataa acccaaagaa cctggaataa cagaggcttc
      841 atcggagata atatcaccaa acatgttgtt ggtaataaca acaccgttta gcttagttgg
      901 tgatttaacc aaaatcatag cagcagagtc gatcaattgg tgctgaacag ttaattgtgg
      961 gaactc
//
