LOCUS       sequence-148996-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCfB2799 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2799
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..794
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(111..139)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      811..1000
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 aacttttttt acttcttgct cattagaaag aaagcatagc aatctaatct aagttttaat
       61 tacaaatctg tcataaaaca atgagcaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcaga ggagagggtg
      181 aaggtgatgc tacaatcgga aaactcaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactc tgacctatgg tgttcaatgc ttttcccgtt
      301 atccggatca catgaaaagg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaacgcac tatatctttc aaagatgacg ggaaatacaa gacgcgtgct gtagtcaagt
      421 ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tactgatttt aaagaagatg
      481 gaaacattct cggacacaaa ctcgagtaca actttaactc acacaatgta tacatcacgg
      541 cagacaaaca aaagaatgga atcaaagcta acttcacagt tcgccacaac gttgaagatg
      601 gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtcgacac aaactgtcct ttcgaaagat cccaacgaaa
      721 agcgtgacca catggtcctt catgagtacg taaatgctgc tgggattaca catggcatgg
      781 atgagctcta caaatgaatc gcgtgcattc atccgctcta accgaaaagg aaggagttag
      841 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
      901 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
      961 actgaaaacc ttgcttgaga aggttttggg acgctcgaag atcgcgtcag ctgaagcttc
     1021 gtacgctgca ggtcgacaac ccttaatata cttc
//
