LOCUS       sequence-149002-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK14. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK14_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             992..1000
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgtctcagg agaggcccac gttctaccgg caggagctga acaagacaat ctgggaggtg
       61 cccgagcgtt accagaacct gtctccagtg ggctctggcg cctatggctc tgtgtgtgct
      121 gcttttgaca caaaaacggg gttacgtgtg gcagtgaaga agctctccag accatttcag
      181 tccatcattc atgcgaaaag aacctacaga gaactgcggt tacttaaaca tatgaaacat
      241 gaaaatgtga ttggtctgtt ggacgttttt acacctgcaa ggtctctgga ggaattcaat
      301 gatgtgtatc tggtgaccca tctcatgggg gcagatctga acaacattgt gaaatgtcag
      361 aagcttacag atgaccatgt tcagttcctt atctaccaaa ttctccgagg tctaaagtat
      421 atacattcag ctgacataat tcacagggac ctaaaaccta gtaatctagc tgtgaatgaa
      481 gactgtgagc tgaagattct ggattttgga ctggctcggc acacagatga tgaaatgaca
      541 ggctacgtgg ccactaggtg gtacagggct cctgagatca tgctgaactg gatgcattac
      601 aaccagacag ttgatatttg gtcagtggga tgcataatgg ccgagctgtt gactggaaga
      661 acattgtttc ctggtacaga ccatattgat cagttgaagc tcattttaag actcgttgga
      721 accccagggg ctgagctttt gaagaaaatc tcctcagagt ctgcaagaaa ctatattcag
      781 tctttgactc agatgccgaa gatgaacttt gcgaatgtat ttattggtgc caatcccctg
      841 gctgtcgact tgctggagaa gatgcttgta ttggactcag ataagagaat tacagcggcc
      901 caagcccttg cacatgccta ctttgctcag taccacgatc ctgatgatga accagtggcc
      961 gatccttatg atcagtcctt tgaaagcagg gacctcctta tagatgagtg gaaaagcctg
     1021 acctatgatg aagtcatcag ctttgtgcca ccaccccttg accaagaaga gatggagtcc
//
