LOCUS       sequence-149011-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of STK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             609..617
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gaagacagtt tgactaagca gcctgaagaa gtttttgatg tattagagaa gcttggagaa
       61 gggtcttatg gaagtgtatt taaagcaata cacaaggaat ccggtcaagt tgtcgcaatt
      121 aaacaagtac ctgttgaatc agatcttcag gaaataatca aagaaatttc cataatgcag
      181 caatgtgaca gcccatatgt tgtaaagtac tatggcagtt attttaagaa tacagacctc
      241 tggattgtta tggagtactg tggcgctggc tctgtctcag acataattag attacgaaac
      301 aagacattaa tagaagatga aattgcaacc attcttaaat ctacattgaa aggactagaa
      361 tatttgcact ttatgagaaa aatacacaga gatataaaag ctggaaatat tctcctcaat
      421 acagaaggac atgcaaaatt ggcagatttt ggagtggctg gtcagttaac agatacaatg
      481 gcaaaacgca atactgtaat aggaactcca ttttggatgg ctcctgaggt gattcaagaa
      541 ataggctata actgtgtggc cgacatctgg tcccttggca ttacttctat agaaatggct
      601 gaaggaaaac ctccttatgc tgatatacat ccaatgaggg ctatttttat gattcccaca
      661 aatccaccac caacattcag aaagccagaa ctttggtccg atgatttcac cgattttgtt
      721 aaaaagtgtt tggtgaagaa tcctgagcag agagctactg caacacaact tttacagcat
      781 ccttttatca agaatgccaa acctgtatca atattaagag acctgatcac agaagctatg
      841 gagatcaaag ctaaaagaca tgaggaacag caacgagaat tggaagagga agaa
//
