LOCUS       sequence-149013-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1G1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1G1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(731..742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agggttggca agaagatagg atgtgggaac ttcggagagc tcagattagg taaaaatctc
       61 tacaccaatg aatatgtagc aatcaaactg gaaccaataa aatcacgtgc tccacagctt
      121 catttagagt acagatttta taaacagctt ggcagtgcag gtgaaggtct cccacaggtg
      181 tattactttg gaccatgtgg gaaatataat gccatggtgc tggagctcct tggccctagc
      241 ttggaggact tgtttgacct ctgtgaccga acatttactt tgaagacggt gttaatgata
      301 gccatccagc tgctttctcg aatggaatac gtgcactcaa agaacctcat ttaccgagat
      361 gtcaagccag agaacttcct gattggtcga caaggcaata agaaagagca tgttatacac
      421 attatagact ttggactggc caaggaatac attgaccccg aaaccaaaaa acacatacct
      481 tatagggaac acaaaagttt aactggaact gcaagatata tgtctatcaa cacgcatctt
      541 ggcaaagagc aaagccggag agatgatttg gaagccctag gccatatgtt catgtatttc
      601 cttcgaggca gcctcccctg gcaaggactc aaggctgaca cattaaaaga gagatatcaa
      661 aaaattggtg acaccaaaag gaatactccc attgaagctc tctgtgagaa ctttccagag
      721 gagatggcaa cctaccttcg atatgtcagg cgactggact tctttgaaaa acctgattat
      781 gagtatttac ggaccctctt cacagacctc tttgaaaaga aaggctacac ctttgactat
      841 gcctatgatt gggttgggag acctattcct actccagtag ggtcagttca cgtagattct
      901 ggtgcatctg caataactcg agaa
//
