LOCUS       sequence-149035-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK01. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK01_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1045..1080
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 atggcggcgg cggcggcggc gggcgcgggc ccggagatgg tccgcgggca ggtgttcgac
       61 gtggggccgc gctacaccaa cctctcgtac atcggcgagg gcgcctacgg catggtgtgc
      121 tctgcttatg ataatgtcaa caaagttcga gtagctatca agaaaatcag cccctttgag
      181 caccagacct actgccagag aaccctgagg gagataaaaa tcttactgcg cttcagacat
      241 gagaacatca ttggaatcaa tgacattatt cgagcaccaa ccatcgagca aatgaaagat
      301 gtatatatag tacaggacct catggaaaca gatctttaca agctcttgaa gacacaacac
      361 ctcagcaatg accatatctg ctattttctc taccagatcc tcagagggtt aaaatatatc
      421 cattcagcta acgttctgca ccgtgacctc aagccttcca acctgctgct caacaccacc
      481 tgtgatctca agatctgtga ctttggcctg gcccgtgttg cagatccaga ccatgatcac
      541 acagggttcc tgacagaata tgtggccaca cgttggtaca gggctccaga aattatgttg
      601 aattccaagg gctacaccaa gtccattgat atttggtctg taggctgcat tctggcagaa
      661 atgctttcta acaggcccat ctttccaggg aagcattatc ttgaccagct gaaccacatt
      721 ttgggtattc ttggatcccc atcacaagaa gacctgaatt gtataataaa tttaaaagct
      781 aggaactatt tgctttctct tccacacaaa aataaggtgc catggaacag gctgttccca
      841 aatgctgact ccaaagctct ggacttattg gacaaaatgt tgacattcaa cccacacaag
      901 aggattgaag tagaacaggc tctggcccac ccatatctgg agcagtatta cgacccgagt
      961 gacgagccca tcgccgaagc accattcaag ttcgacatgg aattggatga cttgcctaag
     1021 gaaaagctca aagaactaat ttttgaagag actgctagat tccagccagg atacagatct
//
